IL-10 Protein (AA 19-178) (His tag)

Catalog No. ABIN7596225

Recombinant IL-10 protein expressed in Baculovirus infected Insect Cells.

Quick Overview for IL-10 Protein (AA 19-178) (His tag) (ABIN7596225)

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Baculovirus infected Insect Cells

Application: SDS-PAGE (SDS), Activity Assay (AcA)

Purity: > 95% by SDS-PAGE

Product Details

Protein Characteristics: AA 19-178

Purification tag / Conjugate: This IL-10 protein is labelled with His tag.

Sequence: SPGQGTQSEN SCTHFPGNLP NMLRDLRDAF SRVKTFFQMK DQLDNLLLKE SLLEDFKGYL GCQALSEMIQ FYLEEVMPQA ENQDPDIKAH VNSLGENLKT LRLRLRRCHR FLPCENKSKA VEQVKNAFNK LQEKGIYKAM SEFDIFINYI EAYMTMKIRN

Endotoxin Level: < 1 EU per 1ug of protein (determined by LAL method)

Biological Activity Comment: Measured in a cell proliferation assay using MC/9 mouse mast cells. The ED50 range ≤5ng/ml.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.

Target Details

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Alternative Name: IL-10

Background: IL-10, also known as Interleukin-10, is an anti-inflammatory cytokine. It inhibits the synthesis of pro-inflammatory cytokines like IFN-gamma, IL2, IL3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. This protein has effects in immunoregulation and inflammation and down-regulates the expression of Th1 cytokines, MHC class II antigens, and costimulatory molecules on macrophages. It acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. This protein controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling. Recombinant human IL-10, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Molecular Weight: 19.4 kDa (166aa)

NCBI Accession: NP_000563

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints