IL-21 Protein (AA 18-146)

Catalog No. ABIN7596303

Recombinant IL-21 protein expressed in Escherichia coli (E. coli).

Quick Overview for IL-21 Protein (AA 18-146) (ABIN7596303)

Target: IL-21 (IL21) (Interleukin 21 (IL21))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS), Activity Assay (AcA)

Purity: > 90% by SDS - PAGE

Product Details

Protein Characteristics: AA 18-146

Sequence: MHKSSPQGPD RLLIRLRHLI DIVEQLKIYE NDLDPELLSA PQDVKGHCEH AAFACFQKAK LKPSNPGNNK TFIIDLVAQL RRRLPARRGG KKQKHIAKCP SCDSYEKRTP KEFLERLKWL LQKMIHQHLS

Endotoxin Level: < 1 EU per 1ug of protein (determined by LAL method)

Biological Activity Comment: The activity is determined by the IFN-g ELISA in a using NK-92 human natural killer cells. The ED50 range ≤ 100 ng/ml.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.

Target Details

Target: IL-21 (IL21) (Interleukin 21 (IL21))

Alternative Name: IL-21 Protein

Background: IL-21, also known as Interleukin-21, is produced from activated helper T cells such as Th17 cells and is a member of the common-γ chain family of cytokines that includes IL-2, IL-4, IL-7, IL-9, and IL-15. Previous studies have shown that IL-21 plays an important role in the regulation of both innate and adaptive immune responses. IL-21 is critical for NK cell activation and expansion. IL-21 also promotes terminal B cell differentiation into plasma cells and antibody production. Furthermore, IL-21 enhances antigen-specific CD8 T cell expansion and the differentiation of follicular helper T cells in vivo. IL-21 also promotes the differentiation of the inflammatory Th17 lineage and contributes to autoimmunity. IL-21 isoform is an alternative splice variant form of IL-21 that we previously identified and reported to have a similar function as original IL-21. Follicular helper T cells also produce IL-21 to activate and differentiate B cells. Recombinant mouse IL-21, was expressed in E. coli and purified by using conventional chromatography techniques.

Molecular Weight: 15 kDa (130aa), confirmed by MALDI-TOF

NCBI Accession: NP_068554

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response