Sonic Hedgehog Protein (SHH) (AA 24-197)

Catalog No. ABIN7596444

Recombinant Sonic Hedgehog protein expressed in Escherichia coli (E. coli).

Quick Overview for Sonic Hedgehog Protein (SHH) (AA 24-197) (ABIN7596444)

Target: Sonic Hedgehog (SHH)

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 95% by SDS-PAGE

Product Details

Protein Characteristics: AA 24-197

Sequence: MIIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKISRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG

Endotoxin Level: < 1 EU per 1ug of protein (determined by LAL method)

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.

Target Details

Target: Sonic Hedgehog (SHH)

Alternative Name: Sonic Hedgehog/Shh (C24II)

Background: SHH, also known as Sonic hedgehog protein, is one of three proteins in the mammalian signaling pathway family called hedgehog. It plays a key role in regulating vertebrate organogenesis, such as in the growth of digits on limbs and organization of the brain. It controls cell division of adult stem cells and has been implicated in development of some cancers. Recombinant human SHH was expressed in E. coli and purified by using conventional chromatography techniques.

Molecular Weight: 19.8 kDa (176aa) confirmed by MALDI-TOF

NCBI Accession: NP_000184

Pathways: Hedgehog Signaling, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development