CLIC5 Protein (DYKDDDDK Tag,Strep Tag)
Quick Overview for CLIC5 Protein (DYKDDDDK Tag,Strep Tag) (ABIN7597052)
Target
See all CLIC5 ProteinsProtein Type
Origin
Source
Application
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Purification tag / Conjugate
- This CLIC5 protein is labelled with DYKDDDDK Tag,Strep Tag.
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Purpose
- Human CLIC5-Strep full length protein-synthetic nanodisc
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Chloride Intracellular Channel 5 (CLIC5) (AA 1-251) protein (His tag)
custom-made CLIC5 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Chloride Intracellular Channel 5 (CLIC5) (AA 1-410) protein (His tag)custom-made CLIC5 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Chloride Intracellular Channel 5 (CLIC5) (AA 1-236) protein (His tag)CLIC5 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Chloride Intracellular Channel 5 (CLIC5) (AA 91-190) protein (GST tag)CLIC5 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Chloride Intracellular Channel 5 (CLIC5) (Transcript Variant 1) protein (His tag)CLIC5 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Chloride Intracellular Channel 5 (CLIC5) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)CLIC5 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Comment
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Advantages:
- Highly purified membrane proteins
- High solubility in aqueous solutions
- High stability
- Proteins are in a native membrane environment and remain biologically active
- No detergent and can be used for cell-based assays
- No MSP backbone proteins
- Mammalian cell expression system ensures post- translational modifications
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Buffer
- Solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
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Storage
- -20 °C,-80 °C
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Storage Comment
- Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
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Expiry Date
- 12 months
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- CLIC5 (Chloride Intracellular Channel 5 (CLIC5))
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Alternative Name
- CLIC5
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Background
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DFNB102, DFNB103, MST130, MSTP130
This gene encodes a member of the chloride intracellular channel (CLIC) family of chloride ion channels. The encoded protein associates with actin-based cytoskeletal structures and may play a role in multiple processes including hair cell stereocilia formation, myoblast proliferation and glomerular podocyte and endothelial cell maintenance. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011] -
Molecular Weight
- The human full length CLIC5-Strep protein has a MW of 46.5 kDa
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UniProt
- Q9NZA1
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Pathways
- Sensory Perception of Sound
Target
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