FCGR3A Protein (AA 17-206) (Fc Tag)

Catalog No. ABIN7853282

Recombinant FCGR3A protein expressed in HEK-293 Cells.

Quick Overview for FCGR3A Protein (AA 17-206) (Fc Tag) (ABIN7853282)

Target: FCGR3A (Fc Fragment of IgG, Low Affinity IIIa, Receptor (CD16a) (FCGR3A))

Protein Type: Recombinant

Origin: Cynomolgus

Source: HEK-293 Cells

Application: Affinity Measurement (AM), Antibody Drug Assay (ADA), Immunoassay (IA), Screening Assay (ScA)

Purity: The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 17-206

Purification tag / Conjugate: This FCGR3A protein is labelled with Fc Tag.

Purpose: Recombinant Cynomolgus FCGR3A protein with C-terminal human Fc tag

Specificity: FCGR3A(Gly17-Gly206) hFc(Glu99-Ala330)

Characteristics: Extracellular Domain Protein

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: FCGR3A (Fc Fragment of IgG, Low Affinity IIIa, Receptor (CD16a) (FCGR3A))

Alternative Name: FCGR3A

Background: CD16, FCG3, CD16A, FCGR3, IGFR3, IMD20, FCR-10, FCRIII, CD16-II, FCGRIII, FCRIIIA, FcGRIIIA
This gene encodes a receptor for the Fc portion of immunoglobulin G, and it is involved in the removal of antigen-antibody complexes from the circulation, as well as other responses, including antibody dependent cellular mediated cytotoxicity and antibody dependent enhancement of virus infections. This gene (FCGR3A) is highly similar to another nearby gene (FCGR3B) located on chromosome 1. The receptor encoded by this gene is expressed on natural killer (NK) cells as an integral membrane glycoprotein anchored through a transmembrane peptide, whereas FCGR3B is expressed on polymorphonuclear neutrophils (PMN) where the receptor is anchored through a phosphatidylinositol (PI) linkage. Mutations in this gene are associated with immunodeficiency 20, and have been linked to susceptibility to recurrent viral infections, susceptibility to systemic lupus erythematosus, and alloimmune neonatal neutropenia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2020]

Molecular Weight: predicted molecular mass of 47.8 kDa after removal of the signal peptide. The apparent molecular mass of cFCGR3A-hFc is 55-70 kDa due to glycosylation.

UniProt: Q8SPW2