PRAME Protein (AA 1-509) (DYKDDDDK Tag)
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- Target See all PRAME Proteins
- PRAME (Preferentially Expressed Antigen in Melanoma (PRAME))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-509
- Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This PRAME protein is labelled with DYKDDDDK Tag.
- Purpose
- Recombinant human PRAME Protein with C-terminal 3xFlag tag
- Sequence
- PRAME(Met1-Asn509) 3xFlag tag
- Purity
- The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
- Top Product
- Discover our top product PRAME Protein
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Preferentially Expressed Antigen in Melanoma (PRAME) (AA 1-509) protein (His tag)
custom-made PRAME Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Preferentially Expressed Antigen in Melanoma (PRAME) (AA 1-509) protein (GST tag)PRAME Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Preferentially Expressed Antigen in Melanoma (PRAME) (AA 321-420) protein (His-IF2DI Tag)PRAME Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Preferentially Expressed Antigen in Melanoma (PRAME) (AA 1-509), (full length) protein (His tag)PRAME Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
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- Application Notes
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Extracellular Domain Proteins (ECD) can be used as:
- Immunogens for antibody drug development
- Reagents used for CAR-T positive cell monitoring
- Reagents for antibody screening and functional testing
- Reagents for antibody affinity measurement
- Comment
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The protein was made using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
- Expiry Date
- 12 months
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- Target
- PRAME (Preferentially Expressed Antigen in Melanoma (PRAME))
- Alternative Name
- PRAME (PRAME Products)
- Background
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MAPE, OIP4, CT130, OIP-4
This gene encodes an antigen that is preferentially expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. The encoded protein acts as a repressor of retinoic acid receptor, and likely confers a growth advantage to cancer cells via this function. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014] - Molecular Weight
- predicted molecular mass of 60.8 kDa after removal of the signal peptide.
- UniProt
- P78395
- Pathways
- Retinoic Acid Receptor Signaling Pathway, Nuclear Hormone Receptor Binding
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