CD73 Protein (AA 27-549) (His tag)
Quick Overview for CD73 Protein (AA 27-549) (His tag) (ABIN7597428)
Target
See all CD73 (NT5E) ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 27-549
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Purification tag / Conjugate
- This CD73 protein is labelled with His tag.
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Purpose
- Recombinant human CD73 Protein with C-terminal 10xHis tag
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Specificity
- CD73(Trp27-Ser549) 10xHis tag
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Characteristics
- Extracellular Domain Protein
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5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 29-549) (Active) protein (His tag)
NT5E Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 27-554) (Active) protein (His tag)NT5E Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 27-547) (Active) protein (hFc Tag)NT5E Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 90 % as determined by HPLC Active
5'-Nucleotidase, Ecto (CD73) (NT5E) (Active) protein (Fc Tag)NT5E Origin: Human Host: Human Cells Recombinant >95 % as determined by reducing SDS-PAGE. Active
5'-Nucleotidase, Ecto (CD73) (NT5E) (Active) protein (His tag)NT5E Origin: Human Host: Human Cells Recombinant >95 % as determined by reducing SDS-PAGE. Active
5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 27-549) protein (Fc Tag)NT5E Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 27-549) (Active) protein (His tag,AVI tag,Biotin)NT5E Origin: Human Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
5'-Nucleotidase, Ecto (CD73) (NT5E) protein (His tag)NT5E Origin: Rat Host: Human Cells Recombinant > 90 % as determined by reducing SDS-PAGE.
5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 28-264) protein (GST tag)NT5E Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
5'-Nucleotidase, Ecto (CD73) (NT5E) protein (His tag)NT5E Origin: Mouse Host: Human Cells Recombinant > 95 % as determined by SDS-PAGE
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Application Notes
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Extracellular Domain Proteins (ECD) can be used as:
- Immunogens for antibody drug development
- Reagents used for CAR-T positive cell monitoring
- Reagents for antibody screening and functional testing
- Reagents for antibody affinity measurement
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Comment
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The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Please see Certificate of Analysis for specific instructions of reconstitution.
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Buffer
- Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.
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Storage
- -20 °C,-80 °C
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Storage Comment
- Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
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Expiry Date
- 12 months
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- CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))
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Alternative Name
- CD73
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Background
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NT5E, NT, eN, NT5, NTE, eNT, E5NT, CALJA
The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011] -
Molecular Weight
- predicted molecular mass of 59.3 kDa after removal of the signal peptide. The apparent molecular mass of CD73-His is 55-70 kDa due to glycosylation.
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UniProt
- P21589
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Pathways
- Synaptic Membrane, Ribonucleoside Biosynthetic Process
Target
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