CD3G Protein (AA 23-116) (Fc Tag)

Catalog No. ABIN7852956

This Recombinant CD3G protein is expressed in HEK-293 Cells.

Quick Overview for CD3G Protein (AA 23-116) (Fc Tag) (ABIN7852956)

Target: CD3G (CD3g Molecule, gamma (CD3-TCR Complex) (CD3G))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Affinity Measurement (AM), Antibody Drug Assay (ADA), Immunoassay (IA), Screening Assay (ScA)

Purity: The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 23-116

Purification tag / Conjugate: This CD3G protein is labelled with Fc Tag.

Purpose: Recombinant human CD3G Protein with C-terminal human Fc tag

Specificity: CD3G(Gln23-Ser116) hFc(Glu99-Ala330)

Characteristics: Extracellular Domain Protein

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: CD3G (CD3g Molecule, gamma (CD3-TCR Complex) (CD3G))

Alternative Name: CD3G

Background: T3G, IMD17, CD3GAMMA, CD3-GAMMA
The protein encoded by this gene is the CD3-gamma polypeptide, which together with CD3-epsilon, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. Defects in this gene are associated with T cell immunodeficiency. [provided by RefSeq, Jul 2008]

Molecular Weight: predicted molecular mass of 36.8 kDa after removal of the signal peptide. The apparent molecular mass of CD3G-hFc is 35-55 kDa due to glycosylation.

UniProt: P09693

Pathways: TCR Signaling, CXCR4-mediated Signaling Events