ApoE2 Protein (AA 19-317) (His tag)

Catalog No. ABIN7853006

Recombinant ApoE2 protein expressed in HEK-293 Cells.

Quick Overview for ApoE2 Protein (AA 19-317) (His tag) (ABIN7853006)

Target: ApoE2 (apoe2) (Apolipoprotein E2 (apoe2))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Affinity Measurement (AM), Antibody Drug Assay (ADA), Immunoassay (IA), Screening Assay (ScA)

Purity: The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 19-317

Purification tag / Conjugate: This ApoE2 protein is labelled with His tag.

Purpose: Recombinant human APOE2 Protein with C-terminal 6xHis tag

Specificity: APOE2(R176C)(Lys19-His317) 6xHis tag

Characteristics: Extracellular Domain Protein

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: ApoE2 (apoe2) (Apolipoprotein E2 (apoe2))

Alternative Name: APOE2

Background: APOE2
The protein encoded by this gene is a major apoprotein of the chylomicron. It binds to a specific liver and peripheral cell receptor, and is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. This gene maps to chromosome 19 in a cluster with the related apolipoprotein C1 and C2 genes. Mutations in this gene result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants. [provided by RefSeq, Jun 2016]

Molecular Weight: predicted molecular mass of 35.0 kDa after removal of the signal peptide. The apparent molecular mass of APOE2-His is 25-55 kDa due to glycosylation.

UniProt: P02649