SEMA3F Protein (AA 19-775, R583A, R586A) (His-Fc tag)

Catalog No. ABIN7678283

Recombinant SEMA3F protein expressed in Mammalian Cells.

Quick Overview for SEMA3F Protein (AA 19-775, R583A, R586A) (His-Fc tag) (ABIN7678283)

Target: SEMA3F (Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3F (SEMA3F))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Mammalian Cells

Application: SDS-PAGE (SDS), Western Blotting (WB), Immunogen (Imm), ELISA, Activity Assay (AcA)

Purity: >90 % as determined by SDS-PAGE.

Product Details

Protein Characteristics: AA 19-775, R583A, R586A

Purification tag / Conjugate: This SEMA3F protein is labelled with His-Fc tag.

Purpose: Recombinant Mouse SEMA3F/Semaphorin 3F Protein, C-hFc-His (Active)

Endotoxin Level: <0.1 EU/μg of the protein by the LAL method.

Biological Activity Comment: Measured by its ability to inhibit proliferation of the human lung carcinoma cells (A549). The ED50 for this effect is 40-120 ng/mL.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from a solution in PBS pH 7.4, 5 % Trehalose, 5 % Mannitol.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at -20 to -80°C for twelve months from the date of receipt.

Expiry Date: 12 months

Target Details

Target: SEMA3F (Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3F (SEMA3F))

Alternative Name: Semaphorin-3F

Background: Semaphorin-3F, Semaphorin IV (Sema IV), Sema3f

UniProt: O88632

Pathways: Regulation of Cell Size