EGF Protein (AA 977-1029)

Catalog No. ABIN7607229

Recombinant EGF protein expressed in Escherichia coli (E. coli).

Quick Overview for EGF Protein (AA 977-1029) (ABIN7607229)

Target: EGF (Epidermal Growth Factor (EGF))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS), Western Blotting (WB), Immunogen (Imm), ELISA, Activity Assay (AcA)

Purity: >90 % as determined by SDS-PAGE.

Product Details

Protein Characteristics: AA 977-1029

Purpose: Recombinant Mouse EGF Protein, No tag (Active)

Endotoxin Level: <1 EU/μg of the protein by the LAL method.

Biological Activity Comment: Measured in a cell proliferation assay using mouse embryonic fibroblast cells (Balb/3T3). The ED50 for this effect is typically 0.1-0.6 ng/mL.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from a solution in PBS pH 7.4, 5 % Trehalose, 5 % Mannitol.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at -20 to -80°C for twelve months from the date of receipt.

Expiry Date: 12 months

Target Details

Target: EGF (Epidermal Growth Factor (EGF))

Alternative Name: Urogastrone

Background: Urogastrone, Pro-epidermal growth factor, EGF,

UniProt: P01132

Pathways: NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation