CD3D Protein (AA 22-105) (His tag)

Catalog No. ABIN7678319

Recombinant CD3D protein expressed in Mammalian Cells.

Quick Overview for CD3D Protein (AA 22-105) (His tag) (ABIN7678319)

Target: CD3D (CD3d Molecule, delta (CD3-TCR Complex) (CD3D))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Mammalian Cells

Application: Western Blotting (WB), SDS-PAGE (SDS), Immunogen (Imm), ELISA, Activity Assay (AcA)

Purity: >90 % as determined by SDS-PAGE.

Product Details

Protein Characteristics: AA 22-105

Purification tag / Conjugate: This CD3D protein is labelled with His tag.

Purpose: Recombinant Human CD3D Protein, C-His (Active)

Endotoxin Level: <0.1 EU/μg of the protein by the LAL method.

Biological Activity Comment: Measured by its binding ability in a functional ELISA. When recombinant human CD3D (ABIN7607250 and ABIN7678319) is immobilized at 1.0 μg/mL, 100 μL/well, the concentration of recombinant human CD3E Biotinylated Protein that produces 50% of the optimal binding response is approximately 1.0-6.0 μg/mL.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from a solution in PBS pH 7.4, 5 % Trehalose, 5 % Mannitol.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at -20 to -80°C for twelve months from the date of receipt.

Expiry Date: 12 months

Target Details

Target: CD3D (CD3d Molecule, delta (CD3-TCR Complex) (CD3D))

Alternative Name: T-cell surface glycoprotein CD3 delta chain

Background: T-cell surface glycoprotein CD3 delta chain, CD3D, T-cell receptor T3 delta chain, T3D, CD3d

UniProt: P04234

Pathways: TCR Signaling, CXCR4-mediated Signaling Events