CPE Protein (AA 42-453) (His tag)

Catalog No. ABIN7607349

Recombinant CPE protein expressed in Mammalian Cells.

Quick Overview for CPE Protein (AA 42-453) (His tag) (ABIN7607349)

Target: CPE (Carboxypeptidase E (CPE))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Mammalian Cells

Application: SDS-PAGE (SDS), Western Blotting (WB), ELISA, Immunogen (Imm), Activity Assay (AcA)

Purity: >90 % as determined by SDS-PAGE.

Product Details

Protein Characteristics: AA 42-453

Purification tag / Conjugate: This CPE protein is labelled with His tag.

Purpose: Recombinant Human CPE Protein, C-His (Active)

Endotoxin Level: <0.1 EU/μg of the protein by the LAL method.

Biological Activity Comment: Measured by its ability to cleave a peptide substrate, benzoyl-AR-OH. The product, Arg, is reacted with orthophthaldialdehyde (OPA) to form a fluorescent molecule. The specific activity is >12,000 pmol/min/μg, as measured under the described conditions.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from a solution in PBS pH 7.4, 5 % Trehalose, 5 % Mannitol.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at -20 to -80°C for twelve months from the date of receipt.

Expiry Date: 12 months

Target Details

Target: CPE (Carboxypeptidase E (CPE))

Alternative Name: Prohormone-processing carboxypeptidase

Background: Prohormone-processing carboxypeptidase, Enkephalin convertase, CPE, Carboxypeptidase H, Carboxypeptidase E, CPH

UniProt: P16870

Pathways: Peptide Hormone Metabolism, Synaptic Membrane