CPE Protein (His tag)

Catalog No. ABIN7194612

Product Details

Target: CPE (Carboxypeptidase E (CPE))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This CPE protein is labelled with His tag.

Purpose: Recombinant Human Carboxypeptidase E/CPE Protein (His Tag)(Active)

Sequence: Met 1-Ser 453

Characteristics: A DNA sequence encoding the human carboxypeptidase E (CPE) precursor (NP_001864.1) (Met 1-Ser 453) was fused with a polyhistidine tag at the C-terminus.

Purity: > 90 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Measured by its ability to cleave a peptide substrate, benzoyl-AR-OH. The product, Arg, reacted with orthophthaldialdehyde (OPA) to form a fluorescent molecule. The specific activity is >12,000 pmoles/min/μg.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: CPE (Carboxypeptidase E (CPE))

Alternative Name: Carboxypeptidase E/CPE (CPE Products)

Synonyms: CPE Protein, CPH Protein, Cph-1 Protein, Cph1 Protein, R74677 Protein, fat Protein, CARBE Protein, Cph Protein, si:ch211-198g9.2 Protein, zgc:85981 Protein, carboxypeptidase E Protein, carboxypeptidase E L homeolog Protein, CPE Protein, cpe Protein, Cpe Protein, cpe.L Protein

Background:

Background: Carboxypeptidase E (CPE), also known as Carboxypeptidase H, is a peripheral membrane protein and a zinc metallocarboxypeptidase, and the conversion of proCPE into CPE occurs primarily in secretory vesicles. The active form of CPE cleaves C-terminal amino acid residues of the peptide, and is thus involved in the biosynthesis of peptide hormones and neurotransmitters including insulin, enkephalin, etc. The enzymatic activity is enhanced by millimolar concentrations of Co2+. It has also been proposed that membrane-associated carboxypeptidase E acts as a sorting receptor for targeting regulated secretory proteins which are mostly prohormones and neuropeptides in the trans-Golgi network of the pituitary and in secretory granules into the secretory pathway.Its interaction with glycosphingolipid-cholesterol rafts at the TGN facilitates the targeting. Mutations in this gene are implicated in type I I diabetes due to impaired glucose clearance and insulin resistance.

Synonym: Carboxypeptidase E(CPE for short); Carboxypeptidase H; Enkephalin convertase; Prohormone-processing carboxypeptidase

Molecular Weight: 49.4 kDa

NCBI Accession: NP_001864

Pathways: Peptide Hormone Metabolism, Synaptic Membrane