CPE Protein (Fc Tag)

Catalog No. ABIN7194611

Recombinant CPE protein expressed in HEK-293 Cells.

Quick Overview for CPE Protein (Fc Tag) (ABIN7194611)

Target: CPE (Carboxypeptidase E (CPE))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 85 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This CPE protein is labelled with Fc Tag.

Purpose: Recombinant Human Carboxypeptidase E/CPE Protein (Fc Tag)

Sequence: Met 1-Ser 453

Characteristics: A DNA sequence encoding the human carboxypeptidase E (CPE) precursor (NP_001864.1) (Met 1-Ser 453) was expressed with the C-terminal fused Fc region of human IgG1.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Not validated for activity

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile 100 mM Glycine, 10 mM NaCl, 50 mM Tris, pH 7.5
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: CPE (Carboxypeptidase E (CPE))

Alternative Name: Carboxypeptidase E/CPE

Background: Carboxypeptidase E(CPE for short),Carboxypeptidase H,Enkephalin convertase,Prohormone-processing carboxypeptidase,Carboxypeptidase E (CPE), also known as Carboxypeptidase H, is a peripheral membrane protein and a zinc metallocarboxypeptidase, and the conversion of proCPE into CPE occurs primarily in secretory vesicles. The active form of CPE cleaves C-terminal amino acid residues of the peptide, and is thus involved in the biosynthesis of peptide hormones and neurotransmitters including insulin, enkephalin, etc. The enzymatic activity is enhanced by millimolar concentrations of Co2+. It has also been proposed that membrane-associated carboxypeptidase E acts as a sorting receptor for targeting regulated secretory proteins which are mostly prohormones and neuropeptides in the trans-Golgi network of the pituitary and in secretory granules into the secretory pathway.Its interaction with glycosphingolipid-cholesterol rafts at the TGN facilitates the targeting. Mutations in this gene are implicated in type II diabetes due to impaired glucose clearance and insulin resistance.

Molecular Weight:

Calculated MW: 74.6 kDa

Observed MW: 85-90 kDa

Gene ID: 1363

NCBI Accession: NP_001864

UniProt: P16870

Pathways: Peptide Hormone Metabolism, Synaptic Membrane