GNLY Protein (AA 1-145) (His tag)

Catalog No. ABIN7678445

This Recombinant GNLY protein is expressed in Mammalian Cells.

Quick Overview for GNLY Protein (AA 1-145) (His tag) (ABIN7678445)

Target: GNLY (Granulysin (GNLY))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Mammalian Cells

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Immunogen (Imm), Activity Assay (AcA)

Purity: >90 % as determined by SDS-PAGE.

Product Details

Protein Characteristics: AA 1-145

Purification tag / Conjugate: This GNLY protein is labelled with His tag.

Purpose: Recombinant Human GNLY/Granulysin Protein, C-10*His (Active)

Endotoxin Level: <0.1 EU/μg of the protein by the LAL method.

Biological Activity Comment: Measured by its ability to induce RANTES secretion by human acute monocytic leukemia cells (THP-1). The ED50 for this effect is 0.3-1 μg/mL, in the presence of 10 μg/mL of a crosslinking antibody Mouse Anti-His Antibody.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from a solution in PBS pH 7.4, 5 % Trehalose, 5 % Mannitol.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at -20 to -80°C for twelve months from the date of receipt.

Expiry Date: 12 months

Target Details

Target: GNLY (Granulysin (GNLY))

Alternative Name: T-cell activation protein 519

Background: T-cell activation protein 519, Protein NKG5, TLA519, Lymphokine LAG-2, GNLY, NKG5, Granulysin, LAG2

UniProt: P22749