NBL1 Protein (AA 17-181) (His tag)

Catalog No. ABIN7678507

This Recombinant NBL1 protein is expressed in Mammalian Cells.

Quick Overview for NBL1 Protein (AA 17-181) (His tag) (ABIN7678507)

Target: NBL1 (Neuroblastoma 1, DAN Family BMP Antagonist (NBL1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Mammalian Cells

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Immunogen (Imm), Activity Assay (AcA)

Purity: >90 % as determined by SDS-PAGE.

Product Details

Protein Characteristics: AA 17-181

Purification tag / Conjugate: This NBL1 protein is labelled with His tag.

Purpose: Recombinant Human NBL1 Protein, C-His (Active)

Endotoxin Level: <0.1 EU/μg of the protein by the LAL method.

Biological Activity Comment: Measured by its ability to inhibit BMP-4-induced activity in mouse preosteoblast cells (MC3T3-E1). The ED50 for this effect is 0.2-1 μg/mL in the presence of 50 ng/mL of rhBMP-4.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from a solution in PBS pH 7.4, 5 % Trehalose, 5 % Mannitol.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at -20 to -80°C for twelve months from the date of receipt.

Expiry Date: 12 months

Target Details

Target: NBL1 (Neuroblastoma 1, DAN Family BMP Antagonist (NBL1))

Alternative Name: Protein N03

Background: Protein N03, Neuroblastoma suppressor of tumorigenicity 1, DAND1, DAN domain family member 1, Zinc finger protein DAN, NBL1, DAN

UniProt: P41271