IL12B Protein (AA 23-335) (His tag)
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- Target See all IL12B Proteins
- IL12B (Interleukin 12 beta (IL12B))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 23-335
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Origin
- Mouse
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Source
- Mammalian Cells
- Purification tag / Conjugate
- This IL12B protein is labelled with His tag.
- Application
- SDS-PAGE (SDS), Western Blotting (WB), Immunogen (Imm), ELISA, Activity Assay (AcA)
- Purpose
- Recombinant Mouse IL12B/IL-12 p40/NKSF2 Protein, C-His (Active)
- Purity
- >90 % as determined by SDS-PAGE.
- Endotoxin Level
- <0.1 EU/μg of the protein by the LAL method.
- Biological Activity Comment
- Measured by its ability to inhibit IL-23 induced IL-17 secretion by mouse splenocytes. The ED50 for this effect is 3-18 ng/mL.
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- Lyophilized from a solution in PBS pH 7.4, 5 % Trehalose, 5 % Mannitol.
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at -20 to -80°C for twelve months from the date of receipt.
- Expiry Date
- 12 months
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- Target
- IL12B (Interleukin 12 beta (IL12B))
- Alternative Name
- IL-12 subunit p40 (IL12B Products)
- Background
- IL-12 subunit p40, IL12B, Interleukin-12 subunit beta, IL-12B, CLMF p40, NKSF2, NK cell stimulatory factor chain 2, Cytotoxic lymphocyte maturation factor 40 kDa subunit
- UniProt
- P43432
- Pathways
- JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation
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