FRZB Protein (AA 33-323) (His tag)

Catalog No. ABIN7678557

Recombinant FRZB protein expressed in Mammalian Cells.

Quick Overview for FRZB Protein (AA 33-323) (His tag) (ABIN7678557)

Target: FRZB (Frizzled-Related Protein (FRZB))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Mammalian Cells

Application: SDS-PAGE (SDS), Western Blotting (WB), Immunogen (Imm), ELISA, Activity Assay (AcA)

Purity: >90 % as determined by SDS-PAGE.

Product Details

Protein Characteristics: AA 33-323

Purification tag / Conjugate: This FRZB protein is labelled with His tag.

Purpose: Recombinant Mouse FRZB Protein, C-His (Active)

Endotoxin Level: <0.1 EU/μg of the protein by the LAL method.

Biological Activity Comment: Measured by its ability to inhibit proliferation in mouse preosteoblast cells (MC3T3-E1). The ED50 for this effect is 1.5-6.0 μg/mL.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from a solution in PBS pH 7.4, 5 % Trehalose, 5 % Mannitol.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at -20 to -80°C for twelve months from the date of receipt.

Expiry Date: 12 months

Target Details

Target: FRZB (Frizzled-Related Protein (FRZB))

Alternative Name: Frzb

Background: Frzb, Frezzled, sFRP-3, Fre, Frzb1, Secreted frizzled-related protein 3, Frizzled-related protein 1, Sfrp3, Fiz, Fritz, FrzB-1

UniProt: P97401

Pathways: WNT Signaling, Positive Regulation of fat Cell Differentiation