SEMA3F Protein (AA 19-772, L503M, R585A, R586A, R651A) (His tag)

Catalog No. ABIN7678599

This Recombinant SEMA3F protein is produced in Mammalian Cells.

Quick Overview for SEMA3F Protein (AA 19-772, L503M, R585A, R586A, R651A) (His tag) (ABIN7678599)

Target: SEMA3F (Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3F (SEMA3F))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Mammalian Cells

Application: SDS-PAGE (SDS), Western Blotting (WB), Immunogen (Imm), ELISA, Activity Assay (AcA)

Purity: >90 % as determined by SDS-PAGE.

Product Details

Protein Characteristics: AA 19-772, L503M, R585A, R586A, R651A

Purification tag / Conjugate: This SEMA3F protein is labelled with His tag.

Purpose: Recombinant Human SEMA3F/Semaphorin 3F Protein, N-His (Active)

Endotoxin Level: <0.1 EU/μg of the protein by the LAL method.

Biological Activity Comment: Measured by its binding ability in a functional ELISA. When recombinant human Neuropilin-2 is immobilized at 2 μg/mL, 100 μL/well, it binds recombinant human SEMA3F/Semaphorin 3F (ABIN7607548 and ABIN7678599). The concentration of recombinant human Semaphorin 3F that produces 50% of the optimal binding response is 0.1-0.6 μg/mL.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from a solution in PBS pH 7.4, 5 % Trehalose, 5 % Mannitol.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at -20 to -80°C for twelve months from the date of receipt.

Expiry Date: 12 months

Target Details

Target: SEMA3F (Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3F (SEMA3F))

Alternative Name: Semaphorin-3F

Background: Semaphorin-3F, SEMA3F, Sema IV, Sema III/F, Semaphorin IV

UniProt: Q13275

Pathways: Regulation of Cell Size