CHI3L2 Protein (AA 27-390) (His tag)
Quick Overview for CHI3L2 Protein (AA 27-390) (His tag) (ABIN7678624)
Target
See all CHI3L2 ProteinsProtein Type
Biological Activity
Origin
Source
Application
Purity
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Protein Characteristics
- AA 27-390
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Purification tag / Conjugate
- This CHI3L2 protein is labelled with His tag.
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Purpose
- Recombinant Human CHI3L2 Protein, C-10*His (Active)
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Endotoxin Level
- <0.1 EU/μg of the protein by the LAL method.
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Biological Activity Comment
- Measured by the ability of the immobilized protein to support the adhesion of human squamous cell carcinoma cells (FaDu). When 5 x 10^4 cells per well are added to rhCHI3L2 (ABIN7607573 and ABIN7678624) coated plates for 30 minutes at 37 °C, cell adhesion is enhanced in a dose dependent manner with typical ED50 range of 0.7-2.8 μg/mL. Opitimal dilutions should be determined by each laboratory for each application.
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Chitinase 3-Like 2 (CHI3L2) (AA 1-390) protein (GST tag)
CHI3L2 Origin: Human Host: Wheat germ Recombinant AP, AA, ELISA, WB
Chitinase 3-Like 2 (CHI3L2) (AA 27-390) protein (His-SUMO Tag)CHI3L2 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. SDS
Chitinase 3-Like 2 (CHI3L2) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)CHI3L2 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Chitinase 3-Like 2 (CHI3L2) (Transcript Variant 3) protein (Myc-DYKDDDDK Tag)CHI3L2 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Chitinase 3-Like 2 (CHI3L2) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)CHI3L2 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Buffer
- Lyophilized from a solution in PBS pH 7.4, 5 % Trehalose, 5 % Mannitol.
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at -20 to -80°C for twelve months from the date of receipt.
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Expiry Date
- 12 months
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- CHI3L2 (Chitinase 3-Like 2 (CHI3L2))
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Alternative Name
- CHI3L2
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Background
- CHI3L2, YKL-39, Chondrocyte protein 39, Chitinase-3-like protein 2
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UniProt
- Q15782
Target
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