SEMA3C Protein (AA 21-738, R551A, R552A, R611A, R612A) (Fc Tag)

Catalog No. ABIN7678741

Recombinant SEMA3C protein expressed in Mammalian Cells.

Quick Overview for SEMA3C Protein (AA 21-738, R551A, R552A, R611A, R612A) (Fc Tag) (ABIN7678741)

Target: SEMA3C (Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3C (SEMA3C))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Mammalian Cells

Application: SDS-PAGE (SDS), Western Blotting (WB), Immunogen (Imm), ELISA, Activity Assay (AcA)

Purity: >90 % as determined by SDS-PAGE.

Product Details

Protein Characteristics: AA 21-738, R551A, R552A, R611A, R612A

Purification tag / Conjugate: This SEMA3C protein is labelled with Fc Tag.

Purpose: Recombinant Human SEMA3C Protein, C-hFc (Active)

Endotoxin Level: <0.1 EU/μg of the protein by the LAL method.

Biological Activity Comment: Measured by the ability of the immobilized protein to support the adhesion of mouse vascular endothelial cells (SVEC4-10) to Fibronectin. Recombinant human SEMA3C (ABIN7607694 and ABIN7678741) immobilized at 10 μg/mL, with 2 ng/mL rhFN-1, 100 μL/well, will induce approximately 50-75% adhesion.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from a solution in PBS pH 7.4, 5 % Trehalose, 5 % Mannitol.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at -20 to -80°C for twelve months from the date of receipt.

Expiry Date: 12 months

Target Details

Target: SEMA3C (Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3C (SEMA3C))

Alternative Name: SEMA3C

Background: SEMA3C, Semaphorin-E, Semaphorin-3C, SEMAE, Sema E

UniProt: Q99985