DKK4 Protein (AA 19-224) (His tag)

Catalog No. ABIN7678801

Recombinant DKK4 protein expressed in Mammalian Cells.

Quick Overview for DKK4 Protein (AA 19-224) (His tag) (ABIN7678801)

Target: DKK4 (Dickkopf Homolog 4 (Xenopus Laevis) (DKK4))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Mammalian Cells

Application: SDS-PAGE (SDS), Western Blotting (WB), Immunogen (Imm), ELISA, Activity Assay (AcA)

Purity: >90 % as determined by SDS-PAGE.

Product Details

Protein Characteristics: AA 19-224

Purification tag / Conjugate: This DKK4 protein is labelled with His tag.

Purpose: Recombinant Human DKK4 Protein, C-10*His (Active)

Endotoxin Level: <0.1 EU/μg of the protein by the LAL method.

Biological Activity Comment: Measured by its ability to inhibit Wnt induced TCF reporter activity in human embryonic kidney cells (HEK293). Recombinant human DKK4 (ABIN7607760 and ABIN7678801) inhibits a constant dose of 500 ng/mL of recombinant human Wnt-3a. The ED50 for this effect is 150-900 ng/mL.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from a solution in PBS pH 7.4, 5 % Trehalose, 5 % Mannitol.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at -20 to -80°C for twelve months from the date of receipt.

Expiry Date: 12 months

Target Details

Target: DKK4 (Dickkopf Homolog 4 (Xenopus Laevis) (DKK4))

Alternative Name: DKK4

Background: DKK4, hDkk-4, Dkk-4, Dickkopf-4, Dickkopf-related protein 4

UniProt: Q9UBT3

Pathways: WNT Signaling