EIF4E Protein (AA 1-217)

Catalog No. ABIN7663680

Recombinant EIF4E protein expressed in Escherichia coli (E. coli).

Quick Overview for EIF4E Protein (AA 1-217) (ABIN7663680)

Target: EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: ELISA, Western Blotting (WB)

Purity: Greater than 80 % as determined by SDS-PAGE.

Product Details

Protein Characteristics: AA 1-217

Purpose: Recombinant Human EIF4E

Endotoxin Level: < 1 EU/μg as determined by LAL test.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial at 10000 rpm for 30 s before opening, reconstitute in sterile distilled water to a concentration of 0.1-1 mg/mL by gently pipetting 2-3 times, don't vortex.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 50 mM NaCl, 6 % Trehalose, 0.05 % Tween80, pH 8.0.

Storage: -20 °C,-80 °C

Storage Comment: The lyophilized protein is stable at -20°C for up to 1 year. After reconstitution, the protein solution is stable at -20 to -80°C for 3 months or 1 week at 2 to 8°C under sterile conditions. For extended storage, it is recommended to further dilute in working aliquots, avoid repeated freeze/thaw cycle.

Expiry Date: 12 months

Target Details

Target: EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

Alternative Name: EIF4E

Background: Eukaryotic translation initiation factor 4E, eIF-4E, eIF-4F 25 kDa subunit, mRNA cap-binding protein, EIF4E, EIF4EL1, EIF4F

Molecular Weight: 25 kDa

Pathways: BCR Signaling