14-3-3 sigma/SFN Protein (AA 1-248)

Catalog No. ABIN7665627

This Recombinant 14-3-3 sigma/SFN protein is produced in Escherichia coli (E. coli).

Quick Overview for 14-3-3 sigma/SFN Protein (AA 1-248) (ABIN7665627)

Target: 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB), ELISA

Purity: Greater than 95 % as determined by SDS-PAGE.

Product Details

Protein Characteristics: AA 1-248

Purpose: Recombinant Human Stratifin

Endotoxin Level: < 1 EU/μg as determined by LAL test.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial at 10000 rpm for 30 s before opening, reconstitute in sterile distilled water to a concentration of 0.1-1 mg/mL by gently pipetting 2-3 times, don't vortex.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 250 mM NaCl, 1 mM EDTA, 1 mM DTT, pH 7.4.

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: The lyophilized protein is stable at -20°C for up to 1 year. After reconstitution, the protein solution is stable at -20 to -80°C for 3 months or 1 week at 2 to 8°C under sterile conditions. For extended storage, it is recommended to further dilute in working aliquots, avoid repeated freeze/thaw cycle.

Expiry Date: 12 months

Target Details

Target: 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))

Alternative Name: Stratifin

Background: 14-3-3 Protein Sigma, Epithelial Cell Marker Protein 1, Stratifin, SFN, HME1

Molecular Weight: 30 kDa

UniProt: P31947

Pathways: p53 Signaling, Myometrial Relaxation and Contraction