A33R (Inactive) protein (His tag)

Catalog No. ABIN7668091

This Recombinant A33R protein is produced in Escherichia coli (E. coli).

Quick Overview for A33R (Inactive) protein (His tag) (ABIN7668091)

Target: A33R

Protein Type: Recombinant

Biological Activity: Inactive

Origin: Mpox Virus

Source: Escherichia coli (E. coli)

Purity: > 95 %

Product Details

Purification tag / Conjugate: His tag

Purpose: Recombinant Monkeypox virus A33R protein (His tag)

Sequence: Met 1-Asp 142

Sterility: 0.2 μm filtered

Endotoxin Level: < 10 EU/mg of the protein as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Buffer: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % -8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: A33R

Background: Monkeypox Virus (MPXV), the virus that causes monkeypox infection in both humans and animals, is a double-stranded DNA virus that has had a recent global outbreak in 2022 . MPXV belongs to the Poxviridae family of viruses . It consists of several key subunits including a surface membrane fusion protein (A29L, ~14 kDa), two separate envelope proteins (A30L ~14 kDa and H3L ~32 kDa), an envelope glycoprotein, A35R ~15 kDa), a receptor glycoprotein that mimics IFN-alpha/beta (B16, ~37 kDa), a palmitoylated EEV membrane glycoprotein (C19L, ~35 kDa), a secreted IL-18 binding protein (D6L, ~14 kDa), a cell surface-binding protein (E8L, ~32 kDa), a telomere binding protein (I1L, ~36 kDa), and a subunit required for DNA packaging (L1R, 18 kDa) .,A33R

Molecular Weight:

calculated_mw: 15.5 kDa

observed_mw: 18 kDa

UniProt: F1DJJ9