Annexin V Protein (His tag)

Catalog No. ABIN7668179

This Recombinant Annexin V protein is expressed in Escherichia coli (E. coli).

Quick Overview for Annexin V Protein (His tag) (ABIN7668179)

Target: Annexin V (ANXA5) (Annexin A5 (ANXA5))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Immunogen (Imm)

Purity: > 90 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This Annexin V protein is labelled with His tag.

Purpose: Recombinant Annexin V-EGFP Protein (His Tag)

Sequence: AnnexinV(1Met-320Asp)-(GGGGS)4-EGFP(1Met-239Lys)

Purification: > 90 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: < 10 EU/mg of the protein as determined by the LAL method

Biological Activity Comment: Not validated for activity

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with 5 % Trehalose and 5 % Mannitol.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: Annexin V (ANXA5) (Annexin A5 (ANXA5))

Alternative Name: Annexin V

Background: ANXA5,Anchorin CII,Annexin V,Annexin-5,Calphobindin I,Endonexin II,Lipocortin V,Placental anticoagulant protein 4,Placental anticoagulant protein I,Thromboplastin inhibitor,Unconjugated Annexin V,Vascular anticoagulant-alpha,This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.annexin A5,calcium and phospholipid binding protein,endonexin 2,placental protein 4,anchorin CII collagen receptor of chondrocytes,predominantly expressed in major constituent of growth plate chondrocytes,down-regulated in adult articular cartilage.EGFP can be used to detect apoptosis by flow cytometry or fluorescence microscopy.

Molecular Weight:

Calculated MW: 63.8 kDa

Observed MW: 62-70 kDa

Gene ID: 308

UniProt: P08758, C5MKY7

Pathways: Apoptosis