AMH Protein (AA 447-554) (His-TRX)

Catalog No. ABIN7668182

This Recombinant AMH protein is expressed in Escherichia coli (E. coli).

Quick Overview for AMH Protein (AA 447-554) (His-TRX) (ABIN7668182)

Target: AMH (Anti-Mullerian Hormone (AMH))

Protein Type: Recombinant

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: Immunogen (Imm)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 447-554

Purification tag / Conjugate: This AMH protein is labelled with His-TRX.

Purpose: Recombinant Mouse AMH Protein (TRX,His Tag)

Sequence: Ser447-Val554

Purification: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: < 10 EU/mg of the protein as determined by the LAL method

Biological Activity Comment: Not validated for activity

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with 5 % Trehalose and 5 % Mannitol.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: AMH (Anti-Mullerian Hormone (AMH))

Alternative Name: AMH

Background: Amh,Anti-Muellerian hormone,Muellerian-inhibiting factor,Muellerian-inhibiting substance,Anti-Mullerian hormone (AMH), a member of the TGF-beta superfamily, is produced by granulosa cells (GCs) of preantral and small antral follicles and plays a role in regulating the recruitment of primordial follicles and the FSH-dependent development of follicles. BMP15 up-regulates the transcription of AMH and that the inhibition of p38 MAPK decreases the BMP15-induced expression of AMH and SOX9, suggesting that BMP15 up-regulates the expression of AMH via the p38 MAPK signaling pathway, and this process involves the SOX9 transcription factor. AMH is widely used for assessing ovarian reserve, and it is particularly convenient, because it is thought to have minimal variability throughout the menstrual cycle. Fetal anti-Mullerian hormone (AMH) is responsible for normal male sexual differentiation, and circulating AMH is used as a marker of testicular tissue in newborns with disorders of sex development. Anti-Mullerian hormone (AMH) produced in the developing testis induces the regression of the Mullerian duct, which develops into the oviducts, uterus and upper vagina. As well as other hormone receptors, and a decreased ovarian cortex cell proliferation. These results help understand the inhibitory effects of AMH on follicular development.

Molecular Weight:

Calculated MW: 31.8 kDa

Observed MW: 35 kDa

Gene ID: 11705

UniProt: P27106

Pathways: Negative Regulation of Hormone Secretion