CSK Protein (His-GST)

Catalog No. ABIN7668268

This Recombinant CSK protein is produced in Baculovirus infected Insect Cells.

Quick Overview for CSK Protein (His-GST) (ABIN7668268)

Target: CSK (C-Src tyrosine Kinase (CSK))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Baculovirus infected Insect Cells

Purity: > 90 %

Product Details

Purification tag / Conjugate: This CSK protein is labelled with His-GST.

Purpose: Recombinant Human SRC Kinase/c-SRC Protein (His & GST Tag)

Sequence: Met 1-Leu 536

Purification: > 90 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: The specific activity was determined to be > 80 nmol/min/mg using Poly(Glu:Tyr) 4:1 as substrate.

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied as sterile solution of 50 mM Tris, 100 mM NaCl, pH 8.0, 20 % glycerol, 0.3 mM DTT

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Target Details

Target: CSK (C-Src tyrosine Kinase (CSK))

Alternative Name: SRC Kinase

Background: Proto-oncogene tyrosine-protein kinase SRC is a hydrophobic protein belonging to the SRC family kinase including nine members that is a family of non-receptor tyrosine kinases. SRC protein may exist in different forms: C-SRC and V-SRC. C-SRC is only activated under certain circumstances where it is required such as growth factor signaling, while V-SRC is a constitutively active as opposed to normal SRC (C-SRC). Thus, V-SRC is an instructive example of an oncogene protein kinase whereas C-SRC is a proto-oncogene protein kinase. Inhibition of SRC with NR2A tyrosine phosphorylation mediated by PSD-95 may contribute to the lithium-induced downregulation of NMDA receptor function and provide neuroprotection against excitotoxicity.,ASV,SRC1,c-SRC,p60-Src

Molecular Weight:

calculated_mw: 87.7 kDa

observed_mw: 81 kDa

UniProt: P12931

Pathways: TCR Signaling, EGFR Signaling Pathway, Cell-Cell Junction Organization, CXCR4-mediated Signaling Events