Desmin Protein (DES) (His tag)
Quick Overview for Desmin Protein (DES) (His tag) (ABIN7668649)
Target
See all Desmin (DES) ProteinsProtein Type
Biological Activity
Origin
Source
Purity
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Purification tag / Conjugate
- This Desmin protein is labelled with His tag.
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Purpose
- Recombinant Human DES protein (His tag)
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Sequence
- Ser 2-Leu 470
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Purification
- > 95 % as determined by reducing SDS-PAGE.
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Sterility
- 0.2 μm filtered
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Endotoxin Level
- < 10 EU/mg of the protein as determined by the LAL method.
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Desmin (DES) (AA 1-469) protein (His tag)
custom-made DES Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Desmin (DES) (AA 1-470) protein (His tag)custom-made DES Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Desmin (DES) (AA 142-467) protein (His tag)DES Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Desmin (DES) (AA 361-470) protein (GST tag)DES Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
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Buffer
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Lyophilized from sterile PBS, pH 7.4.
Normally 5 % -8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization. -
Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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Expiry Date
- 12 months
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- Desmin (DES)
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Alternative Name
- DES
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Background
- Desmin is a cytoplasmic protein and belongs to the intermediate filament family. interacts with DST and MTM1. Desmin is only expressed in vertebrates, however homologous proteins are found in many organisms. Desmin is the main intermediate filament in mature skeletal, cardiac and smooth-muscle cells. DES founctions as homopolymers to form a stable intracytoplasmic filamentous network connecting myofibrils to each other and to the plasma membrane. .Defects in DES are cause of the myopathy myofibrillar type 1, cardiomyopathy dilated type 1I, and neurogenic scapuloperoneal syndrome Kaeser type.,DES,Desmin,desmin
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Molecular Weight
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calculated_mw: 51.5 kDa
observed_mw: 58 kDa
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UniProt
- P17661
Target
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