Glycerol Kinase Protein (GK) (AA 2-502) (His tag)
Quick Overview for Glycerol Kinase Protein (GK) (AA 2-502) (His tag) (ABIN7668847)
Target
See all Glycerol Kinase (GK) ProteinsProtein Type
Biological Activity
Origin
Source
Purity
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Protein Characteristics
- AA 2-502
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Purification tag / Conjugate
- This Glycerol Kinase protein is labelled with His tag.
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Purpose
- Recombinant E.coli Glycerol kinase Protein (His Tag)
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Sequence
- Thr2-Glu502
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Purification
- > 90 % as determined by reducing SDS-PAGE.
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Sterility
- 0.2 μm filtered
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Endotoxin Level
- < 10 EU/mg of the protein as determined by the LAL method.
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Glycerol Kinase (GK) (AA 1-559) protein (His tag)
custom-made GK Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Glycerol Kinase (GK) (AA 1-524) protein (GST tag)GK Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Glycerol Kinase (GK) (AA 1-496), (full length) protein (His tag)GK Origin: Thermus thermophilus Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
Glycerol Kinase (GK) (AA 2-502) protein (His tag)GK Origin: E. coli Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
Glycerol Kinase (GK) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)GK Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Glycerol Kinase (GK) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)GK Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
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Buffer
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Lyophilized from sterile PBS, pH 7.4.
Normally 5 % -8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization. -
Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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Expiry Date
- 12 months
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- Glycerol Kinase (GK)
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Alternative Name
- Glycerol kinase
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Background
- The protein encoded by this gene belongs to the FGGY kinase family. This protein is a key Enzyme in the regulation of glycerol uptake and metabolism. It catalyzes the phosphorylation of glycerol by ATP, yielding ADP and glycerol-3-phosphate. Mutations in this gene are associated with glycerol kinase deficiency (GKD). Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2011],GK,glpK
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Molecular Weight
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calculated_mw: 56.1 kDa
observed_mw: 55-58 kDa
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UniProt
- P0A6F3
Target
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