Glycerol Kinase Protein (GK) (AA 2-502) (His tag)

Catalog No. ABIN7505453

Product Details

Target: Glycerol Kinase (GK)

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 2-502

Origin: E. coli

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This Glycerol Kinase protein is labelled with His tag.

Sequence: Thr2-Glu502

Characteristics: A DNA sequence encoding the Escherichia coli Glycerol kinase (2T-502E) was expressed with a polyhistidine tag at the N-terminus and C-terminus.

Purity: >90 % as determined by reducing SDS-PAGE.

Biological Activity Comment: Measured by its ability to transfer phosphate from ATP to glycerol. The specific activity is ≥ 200U/mg protein, as measured under the described conditions.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: Glycerol Kinase (GK)

Alternative Name: Glycerol kinase (GK Products)

Synonyms: GK1 Protein, GKD Protein, D930012N15Rik Protein, GK Protein, CG18374 Protein, Dmel\\CG18374 Protein, dGyk Protein, ASTP Protein, Gyk Protein, gk1 Protein, gk2 Protein, gkd Protein, gyk Protein, Gk Protein, BmGK Protein, DKFZp469P1225 Protein, glycerol kinase Protein, Glycerol kinase 1 Protein, glycerol kinase S homeolog Protein, putative glycerol kinase 3 Protein, ATP:glycerol 3-phosphotransferase; glycerokinase; GK Protein, Glycerol kinase Protein, Fanconi anemia core complex associated protein 100 Protein, GK Protein, Gk Protein, Gk1 Protein, gk.S Protein, LOC705001 Protein, glpK Protein, Spico_1557 Protein, Trebr_0397 Protein, Halhy_5315 Protein, gk Protein, FAAP100 Protein

Background:

Background: Glycerol kinase from E. coli (glpK) catalyzes the ATP-dependent phosphorylation of glycerol to produce sn-glycerol-3-phosphate (G3P), the first and rate-limiting step in the utilization of glycerol. In the presence of glycerol, glpK is stimulated by interaction with the membrane-bound glycerol facilitator. In the presence of glucose, glpK activity is allosterically inhibited by fructose-1,6-bisphosphate (FBP) of the glycolytic pathway. Under physiological conditions, the enzyme is in an equilibrium between the active dimer and the inactive tetramer. FBP binds to and stabilizes the inactive form, therefore shifting the usage of glycerol metabolic pathway to glycolytic pathway. GlpK is a member of a superfamily of ATPases that includes actin, hexokinase and the heat shock protein hsc70. Although these proteins are dissimilar in amino acid sequence and function, they share similar tertiary folds and likely the same catalytic mechanism. The enzyme activity was measured using a phosphatase-coupled kinase assay.

Synonym: GK, glpK

Molecular Weight: 56.1 kDa

UniProt: P0A6F3