GSK3 alpha Protein (AA 97-448) (His tag)

Catalog No. ABIN7668848

This Recombinant GSK3 alpha protein is expressed in Escherichia coli (E. coli).

Quick Overview for GSK3 alpha Protein (AA 97-448) (His tag) (ABIN7668848)

Target: GSK3 alpha (GSK3a) (Glycogen Synthase Kinase 3 alpha (GSK3a))

Protein Type: Recombinant

Biological Activity: Inactive

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 80 %

Product Details

Protein Characteristics: AA 97-448

Purification tag / Conjugate: This GSK3 alpha protein is labelled with His tag.

Purpose: Recombinant Human GSK3α Protein

Sequence: Ser97-Pro448

Sterility: 0.2 μm filtered

Endotoxin Level: < 10 EU/mg of the protein as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Buffer: Lyophilized from sterile PBS, pH 7.4., 5 % trehalose, 5 % mannitol, 0.01 % Tween 80.
Normally 5 %-8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: GSK3 alpha (GSK3a) (Glycogen Synthase Kinase 3 alpha (GSK3a))

Alternative Name: GSK3alpha

Background: Constitutively active protein kinase that acts as a negative regulator in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transcription factors and microtubules, by phosphorylating and inactivating glycogen synthase (GYS1 or GYS2), CTNNB1/beta-catenin, APC and AXIN1.,GSK-3 alpha,GSK3A,Glycogen synthase kinase-3 alpha,Serine/threonine-protein kinase GSK3A

Molecular Weight:

calculated_mw: 39.4 kDa

observed_mw: 39 kDa

UniProt: P49840

Pathways: PI3K-Akt Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, BCR Signaling, Warburg Effect