LacI (AA 1-360) protein (His tag)

Catalog No. ABIN7669193

This Recombinant LacI protein is expressed in Escherichia coli (E. coli).

Quick Overview for LacI (AA 1-360) protein (His tag) (ABIN7669193)

Target: LacI

Protein Type: Recombinant

Origin: E. coli

Source: Escherichia coli (E. coli)

Application: Immunogen (Imm)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 1-360

Purification tag / Conjugate: His tag

Purpose: Recombinant E.coli Lactose Operon Repressor/LacI Protein (His Tag)

Sequence: Met1-Gln360

Purification: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: < 10 EU/mg of the protein as determined by the LAL method

Biological Activity Comment: Not validated for activity

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with 5 % Trehalose and 5 % Mannitol.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: LacI

Background: LacI,Lactose operon repressor,Lactose operon repressor (LacI) contains one HTH lacI-type DNA-binding domain, functions as a homotetramer. Lactose operon repressor as a repressor of the lactose operon, which also as an inducer, binds allolactose. If remove residues 1-59, resulting the loss of DNA-binding activity but retains tetrameric structure and inducer-binding activity. If delete residues 340-360, resulting the loss of tetramer formation, but retains dimer formation, inducer-binding activity, and DNA-binding activity.

Molecular Weight:

Calculated MW: 39.4 kDa

Observed MW: 40 kDa

UniProt: P03023