NOG Protein (Fc Tag)

Catalog No. ABIN7669374

Recombinant NOG protein expressed in HEK-293 Cells.

Quick Overview for NOG Protein (Fc Tag) (ABIN7669374)

Target: NOG (Noggin (NOG))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Application: Cell Culture (CC)

Purity: > 98 %

Product Details

Purification tag / Conjugate: This NOG protein is labelled with Fc Tag.

Purpose: Recombinant Human Noggin protein(Fc Tag)

Sequence: Gln 28-Cys 232

Sterility: 0.2 μm filtered

Endotoxin Level: < 0.1 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measure by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 cells. The ED₅₀ for this effect is <0.05 μg/mL in the presence of 50 ng/mL of recombinant human BMP-4.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: NOG (Noggin (NOG))

Alternative Name: Noggin

Background: Noggin is a secreted protein involved at multiple stages of vertebrate embryonic development including neural induction and is known to exert its effects by inhibiting the bone morphogenetic protein (BMP)-signaling pathway. It binds several BMPs with very high (picomolar) affinities; with a marked preference for BMP2 and BMP4 over BMP7. By binding tightly to BMPs; Noggin prevents BMPs from binding their receptors. Noggin binds the bone morphogenetic proteins (BMP) such as BMP-4 and BMP-7; and inhibits BMP signaling by blocking the molecular interfaces of the binding epitopes for both type I and type II receptors. Interaction of BMP and its antagonist Noggin governs various developmental and cellular processes; including embryonic dorsal-ventral axis; induction of neural tissue; formation of joints in the skeletal system and neurogenesis in the adult brain. Noggin plays a key role in neural induction by inhibiting BMP4; along with other TGF-β signaling inhibitors such as chordin and follistatin. Mouse knockout experiments have demonstrated that noggin also plays a crucial role in bone development; joint formation; and neural tube fusion.,NOG;Noggin;SYM1;SYNS1;SYNS1A;symphalangism 1 (proximal);synostoses (multiple) syndrome 1

Molecular Weight:

calculated_mw: 49.1 kDa

observed_mw: 58 kDa

UniProt: Q13253

Pathways: Stem Cell Maintenance, Tube Formation