Orexin A Protein (OXA) (AA 34-66) (His-TRX)

Catalog No. ABIN7669393

This Recombinant Orexin A protein is produced in Escherichia coli (E. coli).

Quick Overview for Orexin A Protein (OXA) (AA 34-66) (His-TRX) (ABIN7669393)

Target: Orexin A (OXA)

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Immunogen (Imm)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 34-66

Purification tag / Conjugate: This Orexin A protein is labelled with His-TRX.

Purpose: Recombinant Human Orexin-A Protein (TRX,His Tag)

Sequence: Gln34-Leu66

Purification: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: < 10 EU/mg of the protein as determined by the LAL method

Biological Activity Comment: Not validated for activity

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with 5 % Trehalose and 5 % Mannitol.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: Orexin A (OXA)

Alternative Name: Orexin-A

Background: HCRT,Hcrt1,Hypocretin-1,OX,Orexin-A,PPORX,PPOX,Neuropeptides that play a significant role in the regulation of food intake and sleep-wakefulness, possibly by coordinating the complex behavioral and physiologic responses of these complementary homeostatic functions. A broader role in the homeostatic regulation of energy metabolism, autonomic function, hormonal balance and the regulation of body fluids, is also suggested.

Molecular Weight:

Calculated MW: 23.5 kDa

Observed MW: 25 kDa

Gene ID: 3060

UniProt: O43612

Pathways: Synaptic Membrane, Feeding Behaviour