Pleiotrophin Protein (PTN) (His tag)

Catalog No. ABIN7669446

Recombinant Pleiotrophin protein expressed in Escherichia coli (E. coli).

Quick Overview for Pleiotrophin Protein (PTN) (His tag) (ABIN7669446)

Target: Pleiotrophin (PTN)

Protein Type: Recombinant

Biological Activity: Inactive

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 98 %

Product Details

Purification tag / Conjugate: This Pleiotrophin protein is labelled with His tag.

Purpose: Recombinant Human Pleiotrophin protein(N-His)

Sequence: Gly 33-Asp 168

Sterility: 0.2 μm filtered

Endotoxin Level: < 0.01 EU per μg of the protein as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: Pleiotrophin (PTN)

Alternative Name: Pleiotrophin

Background: HB-GAM belongs to the pleiotrophin family. During embryonic and early postnatal development, HB-GAM is expressed in the central and peripheral nervous system and also in several non-neural tissues, notably lung, kidney, gut and bone. While in the adult central nervous system, it is expressed in an activity-dependent manner in the hippocampus where it can suppress long term potentiation induction. HB-GAM has a low expression in other areas of the adult brain, but it can be induced by ischemic insults, or targeted neuronal damaged in the entorhinal cortex or in the substantia nigra pars compacta. It is structurally related to midkine and retinoic acid induced heparin-binding protein and has a high affinity for heparin. HB-GAM binds anaplastic lymphoma kinase (ALK) which induces MAPK pathway activation, an important step in the anti-apoptotic signaling of PTN and regulation of cell proliferation. It also functions as a secreted growth factor and induces neurite outgrowth and which is mitogenic for fibroblasts, epithelial, and endothelial cells.,Heparin Affin Regulatory Protein (HARP),Heparin-Binding Growth Factor-8 (HBGF-8),Osteoblast-Specif ic Factor 1 (OSF-1),PTN

Molecular Weight:

calculated_mw: 19.9 kDa

observed_mw: 18 kDa

UniProt: P21246

Pathways: RTK Signaling