14-3-3 sigma/SFN Protein

Catalog No. ABIN7669788

Recombinant 14-3-3 sigma/SFN protein expressed in Escherichia coli (E. coli).

Quick Overview for 14-3-3 sigma/SFN Protein (ABIN7669788)

Target: 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))

Protein Type: Recombinant

Biological Activity: Inactive

Origin: Mouse

Source: Escherichia coli (E. coli)

Purity: > 95 %

Product Details

Purpose: Recombinant Mouse 14-3-3 sigma/YWHAS Protein

Sequence: Met 1-Ser 248

Purification: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile 50 mM Tris, 150 mM NaCl, pH 8.0
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))

Alternative Name: 14-3-3 sigma

Background: 14-3-3 protein sigma (YWHAS), also known as stratifin (SFN) and epithelial cell marker protein 1, is a member of the14-3-3 proteins which are a family of conserved regulatory molecules expressed in all eukaryotic cells. The name 14-3-3 refers to the particular elution and migration pattern of these proteins on DEAE-cellulose chromatography and starch-gel electrophoresis. The 14-3-3 proteins eluted in the 14th fraction of bovine brain homogenate and were found on positions 3.3 of subsequent electrophoresis. There are seven genes that encode 14-3-3s in most mammals. 14-3-3 proteins have been identified as adapter proteins implicated in the regulation of a large spectrum of both general and specialized signaling pathway. More than 100 signaling proteins have been reported as 14-3-3 ligands including kinases, phosphatases, and transmembrane receptors, and the binding generally results in the modulation of the activity of the binding partner. YWHAE exists as a homodimer and present mainly in tissues enriched in stratified squamous keratinising epithelium. YWHAS has been repoted to interact with KRT17 and GAB2, and may regulate protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway upon binding to KRT17. Additionally, YWHAS (SFN) may also act as a p53-regulated inhibitor of G2/M progression.,Er,Mme1,Ywhas

Molecular Weight:

calculated_mw: 27.7 kDa

observed_mw: 32 kDa

UniProt: O70456

Pathways: p53 Signaling, Myometrial Relaxation and Contraction