VEGF 165 (Active) protein (His tag)

Catalog No. ABIN7669958

This Recombinant protein is expressed in Escherichia coli (E. coli).

Quick Overview for VEGF 165 (Active) protein (His tag) (ABIN7669958)

Target: VEGF 165

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: Cell Culture (CC)

Purity: > 98 %

Product Details

Purification tag / Conjugate: His tag

Purpose: Recombinant Human VEGF165 protein(His Tag)

Sequence: Ala 27-Arg 191

Purification: > 98 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: < 0.1 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measure by its ability to induce HUVEC cells proliferation. The ED50 for this effect is < 5 ng/mL. The specific activity of recombinant human VEGF165 is approximately > 1. 4 x 10⁶IU/mg.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: VEGF 165

Alternative Name: VEGF165

Background: Vascular endothelial growth factor (VEGF), also known as vascular permeability factor (VPF) and VEGF-A, is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is a member of the platelet-derived growth factor (PDGF)/vascular endothelial growth factor (VEGF) family and often exists as a disulfide-linked homodimer. VEGF-A protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, inhibiting apoptosis and tumor growth. VEGF-A protein is also a vasodilator that increases microvascular permeability, thus it was originally referred to as vascular permeability factor.,Folliculostellate cell-derived growth factor,Glioma-derived endothelial cell mitogen,VPF

Molecular Weight:

calculated_mw: 20.1 kDa

observed_mw: 21 kDa

UniProt: P15692-4