CD3E & CD3G (AA 23-126) protein (His tag)

Catalog No. ABIN7829746

Recombinant CD3E & CD3G protein expressed in HEK-293 Cells.

Quick Overview for CD3E & CD3G (AA 23-126) protein (His tag) (ABIN7829746)

Target: CD3E & CD3G

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Affinity Measurement (AM), Antibody Drug Assay (ADA), Immunoassay (IA), Screening Assay (ScA)

Purity: The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 23-126

Purification tag / Conjugate: His tag

Purpose: Recombinant human CD3E-knob protein with C-terminal 6xHis tag and human CD3G-Hole protein with C-Strep tag

Specificity: Knob-CD3E(Asp23-Asp126)+6xHis tag Hole-CD3G(Gln23-Ser116)+Strep tag

Characteristics: Extracellular Domain Protein

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: CD3E & CD3G

Background: T3E, TCRE, IMD18, CD3epsilon and T3G, IMD17, CD3GAMMA, CD3-GAMMA
T-cell surface glycoprotein CD3 delta & CD3 gamma chain, also known as CD3D & CD3G or CD3D&CD3G respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.

Molecular Weight: predicted molecular mass of 39.2 kDa and 40.0 kDa after removal of the signal peptide.