Ephrin B1 Protein (EFNB1) (AA 28-237) (His tag)

Catalog No. ABIN7852828

This Recombinant Ephrin B1 protein is produced in HEK-293 Cells.

Quick Overview for Ephrin B1 Protein (EFNB1) (AA 28-237) (His tag) (ABIN7852828)

Target: Ephrin B1 (EFNB1)

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Affinity Measurement (AM), Antibody Drug Assay (ADA), Immunoassay (IA), Screening Assay (ScA)

Purity: The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 28-237

Purification tag / Conjugate: This Ephrin B1 protein is labelled with His tag.

Purpose: Recombinant human EFNB1 Protein with C-terminal 6xHis tag

Specificity: EFNB1(Leu28-Lys237) 6xHis tag

Characteristics: Extracellular Domain Protein

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: Ephrin B1 (EFNB1)

Alternative Name: EFNB1

Background: CFND, CFNS, EFB1, EFL3, EPLG2, Elk-L, LERK2
The protein encoded by this gene is a type I membrane protein and a ligand of Eph-related receptor tyrosine kinases. It may play a role in cell adhesion and function in the development or maintenance of the nervous system. [provided by RefSeq, Jul 2008]

Molecular Weight: predicted molecular mass of 23.8 kDa after removal of the signal peptide. The apparent molecular mass of EFNB1-His is 25-55 kDa due to glycosylation.

UniProt: P98172

Pathways: RTK Signaling