IgE Protein
Quick Overview for IgE Protein (ABIN7855496)
Target
See all IgE ProteinsProtein Type
Origin
Source
Application
Purity
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Specificity
- 4-Hydroxy-3-nitrophenylacetyl (NP)
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Sensitivity
- 100%, compared to calibrator (Internal Standard IgE (JW8)), tested by SPR (Biacore™) binding to immobilized recombinant human high affinity IgE receptor
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Characteristics
- Clone: NBS-U3361851G0, Isotype: Human FcEpsilon with murine kappa
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Purification
- Affinity - purified from rec. cell culture supernatant by Immunoaffinity Chromatography (Select IgE Affinity Matrix+HiLoad 26/600 Superdex 200pg)
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IgE (AA 209-428) protein (His-Avi Tag)
Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
IgE (AA 209-429) (Active) protein (His-Avi Tag)Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
IgE (AA 91-421) (Active) protein (His tag)Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
IgE (AA 198-421) (Active) protein (His-Avi Tag)Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
IgE protein (Biotin)Origin: Human Host: Human Recombinant > 95 %, tested by analytical Gel Permeation Chromatography WB, ELISA, IsoC, FACS
IgE (AA 1-429) protein (His tag)Origin: Rat Host: Escherichia coli (E. coli) Recombinant > 95 % WB, SDS, Imm, PC
IgE (AA 1-432) protein (His tag)Origin: Cynomolgus Host: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
IgE (AA 1-428) protein (His tag)Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
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Restrictions
- For Research Use only
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Concentration
- 1 mg/mL
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Buffer
- Phosphate buffered saline (PBS); pH = 7.2; 0.1% NaN3 (sodium azide)
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Preservative
- Sodium azide
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Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
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Handling Advice
- Aliquot to Avoid repeated freezing and thawing.
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Storage
- 4 °C/-20 °C/-80 °C
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- IgE
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Target Type
- Antibody
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Background
- Recombinant chimeric IgE consisting of a human Fc heavy chain linked to the antigen binding variable region of a mouse hybridoma by in vitro DNA recombination. The antibody is specific for the hapten 4-Hydroxy-3-nitrophenylacetyl (NP). IgE (JW8) recognizes the human high affinity IgE receptor (FcRI) and does not interact with the corresponding IgE receptors of other species. It can be used as functional reagent in mast cell / basophil cellular assays, e.g. for controlled sensitization of such cells and subsequent cross-linking with NP-BSA conjugate as specific cross-linking agent.
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Gene ID
- 3497
Target
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