NUCB2 Protein
Quick Overview for NUCB2 Protein (ABIN7855719)
Target
See all NUCB2 ProteinsProtein Type
Origin
Source
Application
Purity
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Purpose
- Purified Nucleobindin-2 (NUCB2) CHO-S
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Characteristics
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Protein Production: Nucleobindin-2 CHO-S (NUCB2, UniProt # G3IF52) with a 6xHIS tag was expressed in CHO-S cells and purified by nickel affinity chromatography.
Migration Behaviour: Under reducing conditions, CHO NUCB2 is detected at 3 bands with estimated molecular weight of ~56, 53 and 15 kDa due to in vivo process such as caspase mediated cleavage. Its concentration is determined using a Bradford protein assay BSA standard curve and A280 (with Extinction Coefficients 0.51).
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Purification
- Purified by nickel affinity chromatography.
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Nucleobindin 2 (NUCB2) (AA 25-106) protein (His-IF2DI Tag)
NUCB2 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Nucleobindin 2 (NUCB2) (AA 25-106) protein (His-IF2DI Tag)NUCB2 Origin: Rat Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Nucleobindin 2 (NUCB2) protein (Myc-DYKDDDDK Tag)NUCB2 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Buffer
- PBS, pH 7.2, 0.09 % Sodium azide
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handling Advice
- Following the initial thaw, please prepare multiple aliquots,It is not recommended to store the product in a frost-free freezer,Multiple freeze-thaw cycles are not recommended,Multiple freeze-thaw cycles are not recommended
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Storage
- -20 °C,-80 °C
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Storage Comment
- Store at -20°C to -80°C. The thawed product is stable at 2-8°C for 7 days. Following the initial thaw, please prepare multiple aliquots and store at -20°C to -80°C. It is not recommended to store the product in a frost-free freezer. Multiple freeze-thaw cycles are not recommended.
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Expiry Date
- 12 months
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- NUCB2 (Nucleobindin 2 (NUCB2))
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Alternative Name
- NUCB2
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Molecular Weight
- 15 kDa
Target
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