CDK6/CyclinD3 (Inactive) protein (His tag)
Quick Overview for CDK6/CyclinD3 (Inactive) protein (His tag) (ABIN7856005)
Target
Protein Type
Biological Activity
Origin
Source
Application
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Purification tag / Conjugate
- His tag
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Purpose
- Recombinant CDK6/CyclinD3, Unactive
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Sequence
- Full Length
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Characteristics
- Recombinant full length human CDK6 and CyclinD3 were co-expressed by baculovirus in Sf9 insect cells using N-terminal His tags on both proteins.
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Sterility
- Sterile
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Comment
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Inactive proteins are produced using methods that do not include specific treatments or conditions to activate them enzymatically. Consequently, these proteins are “unactivated” and may exhibit minimal basal activity. Inactive proteins can be utilized as substrates, negative controls, or for studying the activation of specific enzymes in vitro.
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Restrictions
- For Research Use only
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Format
- Liquid
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Buffer
- Supplied as sterile 50 mM sodium phosphate, pH 7.5, 300 mM NaCl, 50-200 mM imidazole, 0-0.1 mM PMSF, 0.2-1.0 mM DTT, 10-25 % glycerol
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Preservative
- Dithiothreitol (DTT), PMSF
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Precaution of Use
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- -80 °C
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Storage Comment
- Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. Product shipped on dry ice.
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Expiry Date
- 12 months
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