CD16 Protein (CD16) (His-Avi Tag,Biotin)

Catalog No. ABIN7865775

This Recombinant CD16 protein is produced in HEK-293 Cells.

Quick Overview for CD16 Protein (CD16) (His-Avi Tag,Biotin) (ABIN7865775)

Target: CD16

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: >95 % , as determined by SDS-PAGE and HPLC

Product Details

Purification tag / Conjugate: This CD16 protein is labelled with His-Avi Tag,Biotin.

Purpose: Biotinylated Human Fc gamma RIIIB/CD16b (NA2) Protein Recombinant

Sequence: Gly17-Ser200

Characteristics:

Protein Size: Recombinant Human Fc gamma RIIIB/CD16b (NA2) is a protein consisting of amino acid residues [Gly17-Ser200] ,The protein has a predicted MW of 23.7 kDa. Due to glycosylation, the protein migrates to 47-53 kDa based on Tris-Bis PAGE result..

Sequence Position: DNA sequence encoding Human Fc gamma RIIIB/CD16b (NA2) including a C-His-Avi tag was expressed in HEK293 Cells. The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.

Sterility: 0.22 μm filtered

Endotoxin Level: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/μg(1EU/μg).

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM

Buffer: Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.

Storage: -20 °C

Storage Comment: The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.

Expiry Date: 24 months

Target Details

Target: CD16

Alternative Name: CD16

Background: CD16, Fc gamma RIIIB, FCG3, FCGR3B, FcgRIIIB, FCRIIIB, IGFR3, CD16b (NA2), CD16B, FCG3B, FCG3, FCGR3, FCR-10

Molecular Weight: 47-53 kDa

UniProt: O75015

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process