PD-L1 Protein (His-Avi Tag,Biotin)
Quick Overview for PD-L1 Protein (His-Avi Tag,Biotin) (ABIN7865780)
Target
See all PD-L1 ProteinsProtein Type
Origin
Source
Purity
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Purification tag / Conjugate
- This PD-L1 protein is labelled with His-Avi Tag,Biotin.
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Purpose
- Biotinylated Human PD-L1/B7-H1 Protein Recombinant
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Sequence
- Phe19-Arg238
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Characteristics
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Protein Size: Recombinant Human PD-L1/B7-H1 is a protein consisting of amino acid residues [Phe19-Arg238] ,The protein has a predicted MW of 28.1 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Tris-Bis PAGE result..
Sequence Position: DNA sequence encoding Human PD-L1/B7-H1 including a C-His-Avi tag was expressed in HEK293 Cells. The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
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Sterility
- 0.22 μm filtered
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Endotoxin Level
- Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/μg(1EU/μg).
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CD274 (PD-L1) (AA 19-238) (Active) protein (Biotin,His-Avi Tag)
PD-L1 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
CD274 (PD-L1) (AA 19-238) protein (His tag,mFc Tag)PD-L1 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ScA, IA, ADA, AM
CD274 (PD-L1) (AA 19-238) (Active) protein (His tag)PD-L1 Origin: Cynomolgus, Rhesus Monkey Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
CD274 (PD-L1) (AA 19-238) (Active) protein (mFc Tag)PD-L1 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
CD274 (PD-L1) (AA 19-238) (Active) protein (Biotin,His tag)PD-L1 Origin: Cynomolgus, Rhesus Monkey Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
CD274 (PD-L1) (AA 19-238) (Active) protein (hFc Tag)PD-L1 Origin: Cynomolgus, Rhesus Monkey Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
CD274 (PD-L1) (AA 19-238) (Active) protein (Biotin,His tag)PD-L1 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
CD274 (PD-L1) (AA 19-238) (Active) protein (His tag,AVI tag,Biotin)PD-L1 Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
CD274 (PD-L1) (AA 18-238) protein (His-DYKDDDDK-Strep II Tag)PD-L1 Origin: Human Host: HEK-293T Cells Recombinant WB, SDS, ELISA, PI
CD274 (PD-L1) (AA 19-238) (Active) protein (Fc Tag)PD-L1 Origin: Mouse Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
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Buffer
- Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
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Storage
- -20 °C
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Storage Comment
- The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
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Expiry Date
- 24 months
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- PD-L1 (CD274 (PD-L1))
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Alternative Name
- CD274
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Background
- CD274, PDL1, PD-L1, PD-L1B7 homolog 1,B7-H, B7H1, B7-H1, PDCD1L1, PDCD1LG1
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Molecular Weight
- 35-45 kDa
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UniProt
- Q9NZQ7
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Pathways
- Cancer Immune Checkpoints
Target
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