CD3 Protein (CD3) (hFc-Avi Tag,Biotin)

Catalog No. ABIN7865786

Recombinant CD3 protein expressed in HEK-293 Cells.

Quick Overview for CD3 Protein (CD3) (hFc-Avi Tag,Biotin) (ABIN7865786)

Target: CD3

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: >95 % , as determined by SDS-PAGE and HPLC

Product Details

Purification tag / Conjugate: This CD3 protein is labelled with hFc-Avi Tag,Biotin.

Purpose: Biotinylated Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein Recombinant

Sequence: Asp23-Asp126(CD3E) & Phe22-Ala105(CD3D)

Characteristics:

Protein Size: Recombinant Human CD3E&CD3D/CD3 epsilon&CD3 delta is a protein consisting of amino acid residues [Asp23-Asp126(CD3E) & Phe22-Ala105(CD3D)] ,The protein has a predicted MW of 40 kDa (CD3E) and 35.4 kDa (CD3D). Due to glycosylation,the protein migrates to 48-60 kDa based on Tris-Bis PAGE result..

Sequence Position: DNA sequence encoding Human CD3E&CD3D/CD3 epsilon&CD3 delta including a C-hFc-Avi tag was expressed in HEK293 Cells. The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.

Sterility: 0.22 μm filtered

Endotoxin Level: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/μg(1EU/μg).

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM

Buffer: Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C

Storage Comment: The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.

Expiry Date: 24 months

Target Details

Target: CD3

Alternative Name: CD3

Background: CD3, CD3e, CD3E, CD3d, T3D, CD3D, CD3E&CD3D, CD3 delta&CD3 epsilon

Molecular Weight: 48-60 kDa

UniProt: P07766

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway