CD80 Protein (CD80) (His-Avi Tag,Biotin)
Quick Overview for CD80 Protein (CD80) (His-Avi Tag,Biotin) (ABIN7865796)
Target
See all CD80 ProteinsProtein Type
Origin
Source
Purity
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Purification tag / Conjugate
- This CD80 protein is labelled with His-Avi Tag,Biotin.
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Purpose
- Biotinylated Human B7-1/CD80 Protein Recombinant
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Sequence
- Val35-Asn242
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Characteristics
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Protein Size: Recombinant Human B7-1/CD80 is a protein consisting of amino acid residues [Val35-Asn242] ,The protein has a predicted MW of 26.8 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result..
Sequence Position: DNA sequence encoding Human B7-1/CD80 including a C-His-Avi tag was expressed in HEK293 Cells. The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
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Sterility
- 0.22 μm filtered
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Endotoxin Level
- Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/μg(1EU/μg).
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CD80 (CD80) (AA 35-242) (Active) protein (Biotin,His-Avi Tag)
CD80 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
CD80 (CD80) (AA 35-242) (Active) protein (His-Avi Tag)CD80 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
CD80 (CD80) (AA 35-242) protein (His tag,mFc Tag)CD80 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ScA, IA, ADA, AM
CD80 (CD80) (AA 35-242) protein (His tag)HPLC verified CD80 Origin: Cynomolgus, Rhesus Monkey Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE.
CD80 (CD80) (AA 137-230) protein (His-DYKDDDDK-Strep II Tag)CD80 Origin: Human Host: HEK-293T Cells Recombinant WB, SDS, ELISA, PI
CD80 (CD80) (AA 35-242) protein (His-DYKDDDDK-Strep II Tag)CD80 Origin: Human Host: HEK-293T Cells Recombinant WB, SDS, ELISA, PI
CD80 (CD80) (AA 38-245) (Active) protein (Fc Tag)CD80 Origin: Mouse Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
CD80 (CD80) (AA 35-242) (Active) protein (Fc Tag,AVI tag,Biotin)CD80 Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Func Active
CD80 (CD80) (AA 35-242) (Active) protein (His tag)CD80 Origin: Human Host: Mammalian Cells Recombinant >90 % as determined by SDS-PAGE. WB, SDS, ELISA, Imm, AcA Active
CD80 (CD80) (AA 35-288) protein (GST tag)CD80 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
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Buffer
- Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
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Storage
- -20 °C
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Storage Comment
- The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
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Expiry Date
- 24 months
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- CD80
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Alternative Name
- CD80 molecule
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Background
- CD80 Molecule, B7, B71, B7-1, BB1, CD28LG1, CD80, B7.1, CD28LG1, LAB7
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Molecular Weight
- 50-70 kDa
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UniProt
- P33681
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Pathways
- TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints
Target
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