Sclerostin Protein (SOST) (His-Avi Tag,Biotin)

Catalog No. ABIN7865941

This Recombinant Sclerostin protein is expressed in HEK-293 Cells.

Quick Overview for Sclerostin Protein (SOST) (His-Avi Tag,Biotin) (ABIN7865941)

Target: Sclerostin (SOST)

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: >95 % , as determined by SDS-PAGE and HPLC

Product Details

Purification tag / Conjugate: This Sclerostin protein is labelled with His-Avi Tag,Biotin.

Purpose: Biotinylated Human SOST/Sclerostin Protein Recombinant

Sequence: Gln24-Tyr213

Characteristics:

Protein Size: Recombinant Human SOST/Sclerostin is a protein consisting of amino acid residues [Gln24-Tyr213] ,The protein has a predicted MW of 24.2 kDa. Due to glycosylation,the protein migrates to 28-40 kDa based on Tris-Bis PAGE result..

Sequence Position: DNA sequence encoding Human SOST/Sclerostin including a N-His-Avi tag was expressed in HEK293 Cells. The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.

Sterility: 0.22 μm filtered

Endotoxin Level: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/μg(1EU/μg).

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM

Buffer: Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C

Storage Comment: The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.

Expiry Date: 24 months

Target Details

Target: Sclerostin (SOST)

Alternative Name: sclerostin

Background: Sclerostin, SOST, VBCHsclerosteosis, CDD, DAND6, SOST1, VBCH

Molecular Weight: 28-40 kDa

UniProt: Q9BQB4