Cardiotrophin 2 Protein (CTF2) (AA 23-204)

Catalog No. ABIN7866001

This Recombinant Cardiotrophin 2 protein is expressed in Escherichia coli (E. coli).

Quick Overview for Cardiotrophin 2 Protein (CTF2) (AA 23-204) (ABIN7866001)

Target: Cardiotrophin 2 (CTF2)

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Escherichia coli (E. coli)

Purity: >95 % , as determined by SDS-PAGE and HPLC

Product Details

Protein Characteristics: AA 23-204

Purpose: Mouse CTF2 Protein Recombinant

Sequence: MAPISPSEPI GQAYSLALYM QKNTSALLQT YLQHQGSPFS DPGFSAPELQ LSTLPSAAVS FKTWHAMEDA ERLSRAQGAF LALTQHLQLV GDDQSYLNPG SPILLAQLGA ARLRAQGLLG NMAAIMTALG LPIPPEEDTL GFVPFGASAF ERKCRGYIVT REYGHWTDRA VRDLALLKAK YSA

Characteristics:

Protein Size: Recombinant CTF2 migrates as an approximately 20 kDa protein under reducing conditions in SDS-PAGE.

Sequence Position: Optimized DNA sequence encoding Mouse CTF2 mature chain was expressed in E. Coli. Recombinant Mouse Cardiotrophin-2 encodes amino acid residues 23-204.

Endotoxin Level: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/μg(1EU/μg).

Biological Activity Comment: Determined by a cell proliferation using rat C6 cells. The ED50 for this effect is 20.0-30.0 ng/ml.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

Buffer: CTF2 was Lyophilized from PBS, pH 7.2.

Storage: 4 °C,-20 °C

Storage Comment: The lyophilized protein is stable for at least 2 years from date of receipt at -20°C. Upon reconstitution, this product can be stored in working aliquots at 2°- 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Expiry Date: 24 months

Target Details

Target: Cardiotrophin 2 (CTF2)

Alternative Name: CTF2

Molecular Weight: 20 kDa